Through a longitudinal analysis of three time points in a 9-year follow-up study of 3796 middle-aged and elderly individuals, we identified 86 proteins that are related to age. The association patterns of these proteins with age were consistent in both the discovery and validation sets.

We further conducted statistical regression analysis, correlating these 86 proteins with 32 clinical features and the incidence rates of 14 major age-related chronic diseases. These proteins exhibit a high correlation with healthy aging and related cardiometabolic diseases.